- 生物活性
Dinoprost Tromethamine (Dinolytic, PGF2-alpha tham, Zinoprost, Prostin F2 alpha, Dinoprost Trometamol) 是一种合成的prostaglandin F2 alpha类似物，prostaglandin F2 alpha可通过直接作用于黄体素，刺激子宫肌层活性、使宫颈舒张、抑制黄体类固醇生成、诱导黄体溶解。
- 靶点
FP Receptor
Human Endogenous Metabolite
- 体外研究
Dinoprost tromethamine salt (Prostaglandin F2α tromethamine salt; 1 μM; for 24 hours) induces ER stress, autophagy, and apoptosis in goat luteal cells.
Dinoprost tromethamine salt (1 μM; for 24 hours) significantly increases the expression of GRP78 and UPR sensors.
Apoptosis Analysis
Cell Line:
Goat luteal cells
Concentration:
1 μM
Incubation Time:
For 24 hours
Result:
Significantly increased the apoptotic rate (15.62±3.12%).
Cell Autophagy Assay
Cell Line:
Goat luteal cells
Concentration:
1 μM
Incubation Time:
For 24 hours
Result:
There was extensive overlap between LC3 and LAMP1 in luteal cells and autophagolysosomes were formed in goat luteal cells.
Western Blot Analysis
Cell Line:
Goat luteal cells
Concentration:
1 μM
Incubation Time:
For 24 hours
Result:
The expression of GRP78 and UPR sensors including cleaved ATF6, phosphorylated-EIF2S1, EIF2S1, ATF4, phosphorylated-IRE1, autophagy-related protein LC3-II, and pro-apoptosis factor cleaved Caspase3 increased significantly in the cells.
医药
551-11-1
77-86-1
38562-01-5
以(Z)-7-((1R,2R,3R,5S)-3,5-二羟基-2-((S,E)-3-羟基-1-烯-1-基)环戊基)庚-5-烯酸（前列腺素F2α，式6）和三（羟甲基）氨基甲烷为原料合成地诺前列素氨丁三醇的一般步骤：将150g前列腺素F2α（式6）的油状物与5L乙腈混合，加热至完全溶解。控制反应温度在43至47℃之间，搅拌15分钟。随后进行热过滤，收集滤液，并用乙腈洗涤残渣。将乙腈的总体积调整至21L，合并所有滤液，搅拌约5分钟。在搅拌过程中，向滤液中缓慢加入由49.2g三（羟甲基）氨基甲烷和90ml水配制成的氨丁三醇溶液。将反应混合物加热至5324小时。反应完成后，让反应混合物自然冷却至室温，进行过滤。用乙腈洗涤结晶三次，每次100ml。将所得结晶置于五水磷酸盐干燥剂中，进行真空干燥直至恒重，通常需要5小时以上。最终得到约180g地诺前列素氨丁三醇（7），产率为89%。
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