体外研究
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) shows the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells with an IC
50
of 12.5 μM.
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) induces an increase of cell viability on MCF-7 cells mainly after 72 h of treatment whereas it is slightly cytotoxic on MDA-MB231 cells.
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) increases MCF7 cell population in the S phase and in the G2/M phase, no visible change in cell cycle phases is observed in MDA-MB231 cells.
α-Zearalenol (α-zol) (1-10 μM; 24 hours) reduces the expression of TNF-α, α-ZOL combines with β-ZOL shows an antagonistic effect on inflammation for IL-1β and act synergic for IL-8 at high toxin concentrations.
Cell Proliferation Assay
Cell Line:
MFC-7 cells
Concentration:
6.25 µM-25 µM
Incubation Time:
24 hours
Result:
Observed the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells.
Cell Viability Assay
Cell Line:
Breast cancer cell lines: MCF-7 and MDA-MB231
Concentration:
0.001 μM, 0.1 μM and 10 μM
Incubation Time:
24 hours, 48 hours, 72 hours
Result:
Modified the cell growth in two breast cancer cell lines.
Cell Cycle Analysis
Cell Line:
MCF7 cells, MDA-MB231 cells
Concentration:
0.001 μM, 0.1 μM and 10 μM
Incubation Time:
72 hours
Result:
Observed an increase of cells in cell cycle in MCF7 cells.
RT-PCR
Cell Line:
HepG2 cells
Concentration:
1 μM, 5 μM, 10 μM
Incubation Time:
72 hours
Result:
Induced a decrease of the expression of TNF-α, IL-1β and IL-8, that was in most cases statistically significant for the higher doses.
